A remarkable activity of human leukotriene A(4) hydrolase (LTA4H) toward unnatural amino acids

作者：Byzia Anna; Haeggstroem Jesper Z; Salvesen Guy S; Drag Marcin^*

来源：Amino Acids, 2014, 46(5): 1313-1320.

DOI：10.1007/s00726-014-1694-2

• Aminopeptidase
• Unnatural amino acid
• Fluorogenic substrate
• Protease
• Substrate specificity

Leukotriene A(4) hydrolase (LTA4H--EC 3.3.2.6) is a bifunctional zinc metalloenzyme, which processes LTA(4) through an epoxide hydrolase activity and is also able to trim one amino acid at a time from N-terminal peptidic substrates via its aminopeptidase activity. In this report, we have utilized a library of 130 individual proteinogenic and unnatural amino acid fluorogenic substrates to determine the aminopeptidase specificity of this enzyme. We have found that the best proteinogenic amino acid recognized by LTA4H is arginine. However, we have also observed several unnatural amino acids, which were significantly better in terms of cleavage rate (k (cat)/K (m) values). Among them, the benzyl ester of aspartic acid exhibited a k (cat)/K (m) value that was more than two orders of magnitude higher (1.75 x 10(5) M-1 s(-1)) as compared to l-Arg (1.5 x 10(3) M-1 s(-1)). This information can be used for design of potent inhibitors of this enzyme, but may also suggest yet undiscovered functions or specificities of LTA4H.

• 出版日期2014-5