A New Device for Rapid Isolation by Size and Characterization of Rare Circulating Tumor Cells

作者：Desitter Isabelle; Guerrouahen Bella S; Benali Furet Naoual; Wechsler Janine; Jaenne Pasi A; Kuang Yanan; Yanagita Masahiko; Wang Lilin; Berkowitz Jillian A; Distel Robert J; Cayre Yvon E^*

来源：Anticancer Research, 2011, 31(2): 427-441.

• Circulating tumor cells
• CTCs
• microfiltration by size
• real-time quantitative reverse transcriptase-polymerase chain reaction
• qRT-PCR
• molecular biology
• cytomorphology
• FISH
• cell culture
• lung cancer

Background: Circulating tumor cells (CTCs) likely derive from clones in the primary tumor, suggesting that they can be used for all biological tests applying to the primary cells. Materials and Methods: The ScreenCell (R) devices are single-use and low-cost innovative devices that use a filter to isolate and sort tumor cells by size. Results: The ScreenCell (R) Cyto device is able to isolate rare, fixed, tumor cells, with a high recovery rate. Cells are well preserved morphologically. Immunocytochemistry and FISH assays can be performed directly on the filter. The ScreenCell (R) CC device allows isolation of live cells able to grow in culture. High quality genetic materials can be obtained directly from tumor cells isolated on the ScreenCell (R) MB device filter. Conclusion: Due to their reduced size, versatility, and capacity to isolate CTCs within minutes, the ScreenCell (R) devices may be able to simplify and improve non-invasive access to tumor cells.

• 出版日期2011-2