科研之友
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摘要
Objective-To characterize the L1 gene of papillomaviruses detected in epithelial lesions of cats and to determine the relationship between those L1 gene nucleotide sequences and known L1 gene sequences of human and feline papillomaviruses
Sample Population-10 tissue samples of epithelial lesions from 8 cats
Procedures-DNA was extracted from tissue samples Primers were designed to amplify the L1 gene of papillomaviruses Amplicons of DNA were sequenced nucleotide sequences were compared with known L1 gene nucleotide sequences of papillomaviruses and used for phylogenetic analysis
Results-Tissue samples were obtained from lesions (diagnosed as dysplasia [n = 1] squamous cell carcinoma in situ [3] or squamous cell carcinoma 16]) of the skin (9) and oral mucosa 111 Two amplicons had 99% homology with the L1 gene nucleotide sequence of human papillomavirus type 38b subtype FA125 Another amplicon had 84% homology with the L1 gene nucleotide sequence of human papillomavirus type 80 and was considered to be a new type of papillomavirus Phylogenetic tree analysis revealed that these 3 papillomaviruses were grouped into 2 clades that were not similar to the clades of Fells domesticus papillomavirus type 1 or F domesticus papillomavirus type 2 (FdPV2) The remaining 7 amplicons had 98% to 100% homology with the L1 gene nucleotide sequence of FdPV2 Phylogenetic tree analysis revealed that those 7 papillomaviruses were grouped into a single clade with FdPV2
Conclusions and Clinical Relevance-Results support the likelihood of transmission of papillomaviruses between humans and cats (Am J Vet Res 2010 71 1457-1461)
- 出版日期2010-12
