Antiamylase, Anticholinesterases, Antiglycation, and Glycation Reversing Potential of Bark and Leaf of Ceylon Cinnamon (Cinnamomum zeylanicum Blume) In Vitro

作者：Arachchige Sirimal Premakumara Galbada; Abeysekera Walimuni Prabhashini Kaushalya Mendis; Ratnasooriya Wanigasekera Daya

来源：Evidence-Based Complementary and Alternative Medicine, 2017, 2017: 5076029.

DOI：10.1155/2017/5076029

<jats:p>Ethanol (95%) and dichloromethane : methanol (DCM : M, 1 : 1 v/v) bark extracts (BEs) and leaf extracts (LEs) of authenticated Ceylon cinnamon (CC) were studied for antiamylase, antiglucosidase, anticholinesterases, and antiglycation and glycation reversing potential in bovine serum albumin- (BSA-) glucose and BSA-methylglyoxal models in vitro<jats:italic>. </jats:italic>Further, total proanthocyanidins (TP) were quantified. Results showed significant differences (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mi>p</mml:mi><mml:mo>&lt;</mml:mo><mml:mn fontstyle="italic">0.05</mml:mn></mml:math>) between bark and leaf extracts for the studied biological activities (except antiglucosidase) and TP. BEs showed significantly high (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M2"><mml:mi>p</mml:mi><mml:mo>&lt;</mml:mo><mml:mn fontstyle="italic">0.05</mml:mn></mml:math>) activities for antiamylase (IC<jats:sub>50</jats:sub>: <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M3"><mml:mn fontstyle="italic">214</mml:mn><mml:mo>±</mml:mo><mml:mn fontstyle="italic">2</mml:mn></mml:math>–<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M4"><mml:mn fontstyle="italic">215</mml:mn><mml:mo>±</mml:mo><mml:mn fontstyle="italic">10</mml:mn></mml:math> <jats:italic>μ</jats:italic>g/mL), antibutyrylcholinesterase (IC<jats:sub>50</jats:sub>: <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M5"><mml:mn fontstyle="italic">26.62</mml:mn><mml:mo>±</mml:mo><mml:mn fontstyle="italic">1.66</mml:mn></mml:math>–<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M6"><mml:mn fontstyle="italic">36.09</mml:mn><mml:mo>±</mml:mo><mml:mn fontstyle="italic">0.83</mml:mn></mml:math> <jats:italic>μ</jats:italic>g/mL), and glycation reversing in BSA-glucose model (EC<jats:sub>50</jats:sub>: <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M7"><mml:mn fontstyle="italic">94.33</mml:mn><mml:mo>±</mml:mo><mml:mn fontstyle="italic">1.81</mml:mn></mml:math>–<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M8"><mml:mn fontstyle="italic">107.16</mml:mn><mml:mo>±</mml:mo><mml:mn fontstyle="italic">3.95</mml:mn></mml:math> <jats:italic>μ</jats:italic>g/mL) compared to LEs. In contrast, glycation reversing in BSA-methylglyoxal (EC<jats:sub>50</jats:sub>: ethanol: <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M9"><mml:mn fontstyle="italic">122.15</mml:mn><mml:mo>±</mml:mo><mml:mn fontstyle="italic">6.01</mml:mn></mml:math> <jats:italic>μ</jats:italic>g/mL) and antiglycation in both BSA-glucose (IC<jats:sub>50</jats:sub>: ethanol: <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M10"><mml:mn fontstyle="italic">15.22</mml:mn><mml:mo>±</mml:mo><mml:mn fontstyle="italic">0.47</mml:mn></mml:math> <jats:italic>μ</jats:italic>g/mL) and BSA-methylglyoxal models (IC<jats:sub>50</jats:sub>: DCM : M: <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M11"><mml:mn fontstyle="italic">278.29</mml:mn><mml:mo>±</mml:mo><mml:mn fontstyle="italic">8.55</mml:mn></mml:math> <jats:italic>μ</jats:italic>g/mL) were significantly high (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M12"><mml:mi>p</mml:mi><mml:mo>&lt;</mml:mo><mml:mn fontstyle="italic">0.05</mml:mn></mml:math>) in leaf. Compared to the reference drugs used some of the biological activities were significantly (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M13"><mml:mi>p</mml:mi><mml:mo>&lt;</mml:mo><mml:mn fontstyle="italic">0.05</mml:mn></mml:math>) high (BEs: BChE inhibition and ethanol leaf: BSA-glucose mediated antiglycation), some were comparable (BEs: BSA-glucose mediated antiglycation), and some were moderate (BEs and LEs: antiamylase, AChE inhibition, and BSA-MGO mediated antiglycation; DCM : M leaf: BSA-glucose mediated antiglycation). TP were significantly high (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M14"><mml:mi>p</mml:mi><mml:mo>&lt;</mml:mo><mml:mn fontstyle="italic">0.05</mml:mn></mml:math>) in BEs compared to LEs (BEs and LEs: <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M15"><mml:mn fontstyle="italic">1097.90</mml:mn><mml:mo>±</mml:mo><mml:mn fontstyle="italic">73.01</mml:mn></mml:math>–<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M16"><mml:mn fontstyle="italic">1381.53</mml:mn><mml:mo>±</mml:mo><mml:mn fontstyle="italic">45.93</mml:mn></mml:math> and <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M17"><mml:mn fontstyle="italic">309.52</mml:mn><mml:mo>±</mml:mo><mml:mn fontstyle="italic">2.81</mml:mn></mml:math>–<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M18"><mml:mn fontstyle="italic">434.24</mml:mn><mml:mo>±</mml:mo><mml:mn fontstyle="italic">14.12</mml:mn></mml:math> mg cyanidin equivalents/g extract, resp.). In conclusion, both bark and leaf of CC possess antidiabetic properties and thus may be useful in managing diabetes and its complications.</jats:p>

• 出版日期2017