Design of Plasma Surface-Activated, Electrospun Polylactide Non-Wovens with Improved Cell Acceptance

作者：Wyrwa Ralf^*; Finke Birgit; Rebl Henrike; Mischner Nicole; Quaas Marion; Schaefer Jan; Bergemann Claudia; Nebe J Barbara; Schroeder Karsten; Weltmann Klaus Dieter; Schnabelrauch Matthias

来源：Advanced Engineering Materials, 2011, 13(5): B165-B171.

DOI：10.1002/adem.201080116

Electrospinning is a versatile technique to generate tissue engineering matrices possessing structural features similar to the extracellular matrix. Biodegradable polylactides are well suited for processing by this technique, but their innate hydrophobicity impairs initial protein adsorption and cell adhesion. In this work, therefore, electrospun poly(L-lactide-co-D,L-lactide) (70/30) non-wovens are modified with an ultrathin plasma-polymerized allylamine (PPAAm) coating. Using scanning electron microsocopy (SEM), it is shown that the fiber structure of the non-woven is not affected by the plasma treatment. X-ray photoelectron spectroscopy (XPS) and contact angle measurements of PPAAm-coated non-wovens confirm the presence of nitrogen and oxygen-functional groups in the coating and a hydrophilic nature of the coated non-woven surface. Cell experiments in vitro demonstrate that the PPAAm-coated surface promotes occupancy of the non-woven by human MG-63 osteoblasts accompanied by improved initial cell spreading and filopodia formation along and between the electrospun polylactide fibers. Overall, plasma-assisted incorporation of amino groups into electrospun polylactone non-wovens represents a promising approach to tissue engineering scaffolds with improved cell-material interfaces.

• 出版日期2011-5