Apoferritin-templated biosynthesis of manganese nanoparticles and investigation of direct electron transfer of MnNPs-HsAFr at modified glassy carbon electrode

作者：Rafipour Ronak; Kashanian Soheila; Hashemi Sadegh; Omidfar Kobra; Dolatabadi Jafar Ezzati Nazhad

来源：Biotechnology and Applied Biochemistry, 2017, 64(1): 110-116.

DOI：10.1002/bab.1466

• electron transfer
• horse spleen apoferritin
• manganese nanoparticles
• carbon nanotubes
• chitosan

Manganese nanoparticles (MnNPs) were created within horse spleen apoferritin (HsAFr) cavity nanotemplates. Transmission electron microscopy revealed the particle size to be 6 nm. Intrinsic fluorescence data showed that the mineralization acted as a quencher of the HsAFr fluorescence, and extrinsic fluorescence data revealed that the hydrophobic binding site at the surface of HsAFr was not changed. Finally, the MnNP-HsAFr was immobilized onto multiwalled carbon nanotubes entrapped into chitosan (CS) matrices by through sequential 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide-N-hydroxysuccinimide and glutaraldehyde coupling. The MnNPs-HsAFr immobilized on CNT-CS/GC electrode was characterized by cyclic voltammetry. This charge transfer coefficient () and the exchange current (i(0)) of MnNPs-HsAFr immobilized on modified electrode in 0.1 M phosphate solution (pH 7.5) were found to be 0.57 and 0.48 A, respectively.

• 出版日期2017-2