Development of a biointegrated mandibular reconstruction device consisting of bone compatible titanium fiber mesh scaffold

作者：Hirota Makoto^*; Shima Takaki; Sato Itaru; Ozawa Tomomichi; Iwai Toshinori; Ametani Akihiro; Sato Mitsunobu; Noishiki Yasuharu; Ogawa Takahiro; Hayakawa Tohru; Tohnai Iwai

来源：Biomaterials, 2016, 75: 223-236.

DOI：10.1016/j.biomaterials.2015.09.034

• Mandibular reconstruction
• Scaffold
• Hydroxyapatite coating
• Titanium fiber mesh

Coating biomaterials with a thin hydroxyapatite (HA) was proven effective in enhancing bone compatibility. Segmental bone defects are considered as the most difficult defect to repair in bone regeneration therapy. We developed submicron-thin HA-coated titanium fiber mesh scaffolds to reconstruct immediately loaded segmental mandibular defects and evaluated their bone compatibility in vitro and in vivo. Human osteoblasts attachment, proliferation, and osteocalcin expression in non- and HA-coated scaffolds were evaluated. A 10-mm long segmental bone defect in a rabbit mandibular bone was reconstructed with non- or HA-coated scaffolds, which were removed at 9 and 21 weeks, to evaluate the mechanical strength of the bone-scaffold connection and the bone formation around the scaffold. Expression of osteocalcin was greater in HA-coated scaffolds. In vivo bone formation in HA-coated scaffolds was greater than that in non-coated scaffolds at 21 weeks. Newly formed bone in HA-coated scaffolds mostly restored bone continuity. Scanning electron microscopy identified strong integration of the bone and HA-coated scaffolds. The mechanical strength of the bone-scaffold connection was 3-fold greater in HA-coated scaffolds than that in non-coated scaffolds. These results suggest that a thin HA-coated titanium fiber mesh scaffold is a bone-compatible mandibular reconstruction device in immediately loaded segmental defects.

• 出版日期2016-1