科研之友
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摘要
A simple and rapid reversed-phase HPLC-UV method was developed for the determination of triterpenic acids in the crude extract of Prunellae Spica. Five triterpenic acids were extracted and isolated from R Spica as marker compounds for use in the quality control of herbal medicines. Various solvent extraction techniques were evaluated, and the greatest efficiency was observed with sonication in 100% ethanol. Elemental compositions of the five marker compounds were determined by high-resolution mass spectroscopy. The dynamic range of the HPLC-UV method depended on the specific analyte, and acceptable quantitation was obtained between 10 and 250 mu g mL(-1) for oleanolic acid, between 10 and 300 mu g mL(-1) for ursolic acid, between 3 and 75 mu g mL(-1) for 2 alpha,3 alpha,24-trihydroxyolean-12en-28oic acid, between 5 and 100 mu g mL(-1) for euscaphicacid, and between 5 and 100 mu g mL(-1) for 2 alpha,3 alpha-dihydroxyurs-1 2en-28oic acid. The method was deemed satisfactory by inter- and intra-day validation and exhibited both high accuracy and precision (relative standard deviation <9.4%). Overall limits of quantitation and detection were approximately 0.5-2.5 mu g mL(-1) at a signal-to-noise ratio (SIN) of 3 and were about 3.0-10.0 mu g mL(-1) at a SIN of 10. In addition, principal component analysis (PCA) was performed on the analytical data of 15 different P. Spica samples in order to classify samples collected from different regions.
- 出版日期2009-2-9
