Anti-hyperprolactinemia mechanism of Radix bupleuri extract in rats

作者：Miao, Hui; Miao, Cong-xiu^*; Han, Jing; Li, Na

来源：Tropical Journal of Pharmaceutical Research, 2016, 15(9): 1903-1907.

DOI：10.4314/tjpr.v15i9.13

• Hyperprolactinemia
• Radix bupleuri
• Dopamine D2 receptor
• cAMP/PKA

Purpose: To determine the mechanism underlying the anti-hyperprolactinemia effects of Radix bupleuri extract (RBE) in rats.
Methods: Rats were divided into six groups (n=10 each group): healthy controls, untreated hyperprolactinemic rats, hyperprolactinemic rats treated with bromocriptine (0.6 mg/kg), and hyperprolactinemic rats treated with RBE (4.8, 9.6, or 19.2 g/kg). After 30 days, hypothalamic protein levels of dopamine D2 receptor, protein kinase A (PKA), and cyclic adenosine monophosphate (cAMP) were determined.
Results: Dopamine D2 receptor levels were lower in untreated hyperprolactinemic rats than in healthy controls (p < 0.01), but this decrease was attenuated by RBE (p < 0.05). Elevated PKA levels in untreated hyperprolactinemic rats (0.61 +/- 0.04 mu g/ml, p < 0.01) were decreased by RBE (4.8 g/kg, 0.42 +/- 0.03 mu g/ml, p < 0.05; 9.6 g/kg, 0.33 +/- 0.02 mu g/ml, p < 0.01; 19.2 g/kg, 0.27 +/- 0.03 mu g/ml, p < 0.01). Similarly, elevated cAMP levels in hyperprolactinemic rats (2.4 +/- 0.4 ng/ml) were decreased by RBE (4.8 g/kg, 1.8 +/- 0.3 ng/ml, p < 0.05; 9.6 g/kg, 1.5 +/- 0.3 ng/ml, p < 0.01; 19.2 g/kg, 1.2 +/- 0.2 ng/ml, p < 0.01).
Conclusions: RBE anti-hyperprolactinemia activity is mediated by dopamine D2 receptor signaling via the cAMP/PKA pathway.

• 出版日期2016-9
• 单位长治医学院