A novel colorimetric assay for alpha-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate

作者:Yevglevskis Maksims; Lee Guat L; Nathubhai Amit; Petrova Yoana D; James Tony D; Threadgill Michael D; Woodman Timothy J; Lloyd Matthew D*
来源:Chemical communications, 2017, 53(37): 5087-5090.
DOI:10.1039/c7cc00476a

摘要

alpha-Methylacyl-CoA racemase (AMACR; P504S) regulates branched-chain fatty acid degradation, activates Ibuprofen and is a recognised cancer drug target. A novel, facile colorimetric assay was developed based on elimination of 2,4-dinitrophenolate. The assay was used to test 5 known inhibitors, determining IC50 and K-i values, reversibility and characterizing irreversible inhibition.

  • 出版日期2017-5-7