A new sensitive method for the detection of chloramphenicol in food using time-resolved fluoroimmunoassay

作者：Zhou, Bin; Zhang, Jue; Fan, Jun; Zhu, Lan; Zhang, Yi; Jin, Jian; Huang, Biao^*

来源：European Food Research and Technology, 2015, 240(3): 619-625.

DOI：10.1007/s00217-014-2363-0

• Chloramphenicol (CAP)
• Veterinary drug residues
• Time-resolved fluoroimmunoassay (TRFIA)
• Food

A time-resolved fluoroimmunoassay (TRFIA) technique was developed to detect chloramphenicol (CAP) contamination in food. By using CAP-ovalbumin, anti-CAP antibodies, and europium-labelled goat anti-rabbit antibodies, an indirect, competitive method for CAP-TRFIA was established. The sensitivity was high, with a detection limit of 0.008 mu g/L (8 ppt) for indirect competitive TRFIA formats. Testing showed that the sensitivity of the technique was 2.67 mu g/kg in honey, prawn, and chicken muscle tissues, and 0.32 mu g/L in milk. The detection range was between 0.008 and 100 mu g/L: within this, the intra- and inter-batch coefficients of variation of the CAP-TRFIA method were 6.8 and 13.5 %, respectively. The study suggested that CAP-TRFIA was a simple, sensitive, and cost-effective method of screening large quantities of samples and had good prospects for further application.

• 出版日期2015-3
• 单位江南大学; 江苏省原子医学研究所