A novel ratiometric fluorescent probe for the detection of HOCl based on FRET strategy

作者：Shen, Shi-Li; Zhang, Xiao-Fan; Ge, Yan-Qing; Zhu, Yan; Cao, Xiao-Qun^*

来源：Sensors and Actuators B: Chemical , 2018, 254: 736-741.

DOI：10.1016/j.snb.2017.07.158

• Fluorescent probe
• Ratiometric
• Fluorescence resonance energy transfer
• HOCl
• Bioimaging

A novel ratiometric fluorescent probe (RIM) for HOCl was designed on imidazo[1,5-a]pyridine-rhodamine FRET platform. Upon addition of HOCl, the imidazo[1,5-a]pyridine fluorescence increased and the rhodamine fluorescence increased simultaneously. The ratios (158511465) displayed brilliant HOCl-dependent performance and responded linearly to HOCl. RIM could respond to HOCl rapidly (within 20s) with a low detection limit of 24 nM RIM also exhibited high selectivity, and low cytotoxicity. Moreover, it has been successfully applied in fluorescence imaging in living cells and the results confirmed that the probe could selectively stain lysosomes with excellent photostability.

• 出版日期2018-1
• 单位山东第一医科大学