Acanthamoeba castellanii Neff: In vitro activity against the trophozoite stage of a natural sesquiterpene and a synthetic cobalt(II)-lapachol complex

作者：Martin Navarro Carmen M; Lopez Arencibia Atteneri; Lorenzo Morales Jacob; Oramas Royo Sandra; Hernandez Molina Rita; Estevez Braun Ana; Ravelo Angel G; Valladares Basilio; Pinero Jose E^*

来源：Experimental Parasitology, 2010, 126(1): 106-108.

DOI：10.1016/j.exppara.2009.12.015

• Acanthamoeba
• Lapachol
• Sesquiterpene
• Cyperotundone
• Chemotherapy

In this this study, the in vitro activities of a natural sesquiterpene, alpha-cyperotundone, isolated from the root bark of Maytenus retusa and a cobalt(II)-complex of a natural occurring prenyl hydroxynaphthoquinone (lapachol) were evaluated against the trophozoite stage of Acanthamoeba castellanii Neff using a previously developed colorimetric 96-well microtiter plate assay, based on the oxido-reduction of Alamar Blue (R). The obtained activities showed that these two compounds were able to inhibit the in vitro growth of the amoebae at relatively low concentrations. Further identification of the molecular targets of these products and their effects on acanthamoebae should be determined to evaluate their possible therapeutic use.

• 出版日期2010-9