A p53 enhancer region regulates target genes through chromatin conformations in cis and in trans

作者：Link Nichole; Kurtz Paula; O'Neal Melissa; Garcia Hughes Gianella; Abrams John M^*

来源：Genes & Development, 2013, 27(22): 2433-2438.

DOI：10.1101/gad.225565.113

• genome architecture
• chromosome conformation capture
• p53
• apoptosis
• long-distance gene regulation

We examined how a p53 enhancer transmits regulatory information in vivo. Using genetic ablation together with digital chromosome conformation capture and fluorescent in situ hybridization, we found that a Drosophila p53 enhancer region (referred to as the p53 response element [p53RE]) physically contacts targets in cis and across the centromere to control stress-responsive transcription at these sites. Furthermore, when placed at ectopic genomic positions, fragments spanning this element re-established chromatin contacts and partially restored target gene regulation to mutants lacking the native p53RE. Therefore, a defined p53 enhancer region is sufficient for long-range chromatin interactions that enable multigenic regulation.

• 出版日期2013-11-15