Elucidation of the Molecular Mechanism and Exploration of Novel Therapeutics for Spinocerebellar Ataxia Caused by Mutant Protein Kinase C gamma

作者：Seki Takahiro^*; Adachi Naoko; Abe Seki Nana; Shimahara Takayuki; Takahashi Hideyuki; Yamamoto Kazuhiro; Saito Naoaki; Sakai Norio

来源：Journal of Pharmacological Sciences, 2011, 116(3): 239-247.

DOI：10.1254/jphs.11R04CP

• spinocerebellar ataxia
• protein kinase C gamma
• cerebellar Purkinje cell aggregation
• apoptosis

Spinocerebellar ataxia (SCA) is an inherited neurodegenerative disorder that is characterized by cerebellar atrophy and progressive ataxia and is classified into 31 types by the genetic locus. Recently, missense mutations of PRKCG genes that code protein kinase C gamma (gamma PKC) have been identified as a causal gene of SCA14. To explore the molecular mechanism of SCA14 pathogenesis, we investigated how mutant gamma PKC causes the neurodegeneration of cerebellar Purkinje cells (PCs) by expressing mutant gamma PKC-GFP in cell lines and primary cultured PCs. Mutant gamma PKC was susceptible to aggregation in the cytoplasm, which led to an impairment of the ubiquitin-proteasome system and apoptosis. Furthermore, mutant gamma PKC induced improper dendritic development of cultured PCs in an aggregation-independent manner. Stimulation-induced translocation of mutant gamma PKC in PC dendrites was prominently attenuated by the reduced mobility of oligomerized mutant gamma PKC, which resulted in attenuated signal transduction and the improper morphology of PC dendrites. These findings suggested that the oligomerization and aggregation of mutant gamma PKC caused improper dendritic development and apoptosis of PCs, which led to cerebellar dysfunction and SCA14 pathogenesis. We screened the chemicals that improved these cellular dysfunctions and identified several compounds, including trehalose and Congo red, which could be novel therapeutics for SCA14.

• 出版日期2011-7