Activity of rosuvastatin in tachyzoites of Toxoplasma gondii (RH strain) in HeLa cells

作者：Sanfelice Raquel Arruda; Machado Lais Fernanda; Bosqui Larissa Rodrigues; Miranda Sapla Milena Menegazzo; Tomiotto Pellissier Fernanda; Dalevedo Gabriela de Alcantara; Ioris Dielle; Reis Guilherme Fonseca; Panagio Luciano Aparecido; Navarro Italmar Teodorico; Bordignon Juliano; Conchon Costa Ivete; Pavanelli Wander Rogerio; Almeida Ricardo Sergio; Costa Idessania Nazareth^*

来源：Experimental Parasitology, 2017, 181: 75-81.

DOI：10.1016/j.exppara.2017.07.009

• Rosuvastatin
• Toxoplasmosis
• Statins

Due to the toxicity of conventional medication in toxoplasmosis, some drugs are being studied for treating this infection, such as statins, especially rosuvastatin compound, which is efficient in inhibiting the initial isoprenoid biosynthesis processes in humans and the parasite. The goal of this study was to assess the activity of rosuvastatin in HeLa cells infected with the RH strain of T. gondii. In the experiment, HeLa cells (1 x 10(5)) were infected with tachyzoites of T. gondii (5 x 10(5)). After the experimental infection, we assessed the number of infected cells and the amount of intracellular tachyzoites. In addition, culture supernatants were collected to determine the amount of cytokines by cytometric bead array. We observed that there was no cytotoxicity in the concentrations tested in this cell line. The effect of rosuvastatin showed a significant reduction in both the number of infected cells and the proliferation index of the intracellular parasite, when compared with the conventional treatment combining sulfadiazine and pyrimethamine for toxoplasmosis. There were also reduced levels of cytokines IL-6 and IL-17. Therefore, it was concluded that rosuvastatin exhibited antiproliferative activity. The data presented are significant to promote further studies and the search for alternative treatment for toxoplasmosis.

• 出版日期2017-10