Accurate Purity Analysis of L-Lysine Hydrochloride by Using Neutralization Titration and Uncertainty Evaluation

作者：Yamazaki Taichi^*; Eyama Sakae; Takatsu Akiko

来源：Bunseki Kagaku, 2012, 61(11): 959-962.

• titration
• L-lysine hydrochloride
• purity analysis
• amino acid
• primary method

The quantitative analysis of amino acid is widely used in many analysis fields. Purity evaluations of amino acids are essential to prepare accurate calibration solutions for amino acid analysis. In this paper, the purity determination of L-lysine hydrochloride with using neutralization titration is discussed. Removing hydrogen chloride from a sample solution of L-lysine hydrochloride is important to determine the purity by neutralization titration. For an accurate purity determination of L-lysine hydrochloride, the condition in sample preparation was optimized. Under the optimized conditions, the purity of L-lysine hydrochloride could be determined with 0.03 % to 0.07 % of repeatability. The uncertainty of the measurement could be estimated at about 0.1 %. Moreover, the determined purity value was in good agreement with the purity value from nitrogen analysis. The optimized condition is valid for the purity analysis of lysine hydrochloride.

• 出版日期2012-11