MAP1S controls microtubule stability throughout the cell cycle in human cells

作者：Tegha Dunghu Justus; Bausch Elena; Neumann Beate; Wuensche Annelie; Walter Thomas; Ellenberg Jan; Gruss Oliver J^*

来源：Journal of Cell Science, 2014, 127(23): 5007-5013.

DOI：10.1242/jcs.136457

• Microtubule
• Microtubule-associated protein
• MAP1S
• Microtubule dynamics
• Cytokinesis

Understanding the molecular basis for proper cell division requires a detailed functional analysis of microtubule (MT)-associated proteins. MT-associated protein 1S (MAP1S), the most ubiquitously expressed MAP1 family member, is required for accurate cell division. Here, using quantitative analysis of MT plus-end tracking, we show that MAP1S knockdown alters MT dynamics throughout the cell cycle. Surprisingly, MAP1S downregulation results in faster growing, yet short-lived, MTs in all cell cycle stages and in a global loss of MT acetylation. These aberrations correlate with severe defects in the final stages of cell division. In monopolar cytokinesis assays, we demonstrate that MAP1S guides MT-dependent initiation of cytokinesis. Our data underline the key role of MAP1S as a global regulator of MT stability and demonstrate a new primary function of MAP1S to regulate MT dynamics at the onset of cytokinesis.

• 出版日期2014-12-1