A novel gene STYK1/NOK is upregulated in estrogen receptor-alpha negative estrogen receptor-beta positive breast cancer cells following estrogen treatment

作者：Kimbro K Sean^*; Duschene Kaitlin; Willard Margeret; Moore Jodi Ann; Freeman Shalonda

来源：Molecular Biology Reports, 2008, 35(1): 23-27.

DOI：10.1007/s11033-006-9047-1

• breast cancer
• estrogen
• estrogen receptor (ER)
• protein kinase

The human STYK1/NOK protein is approximately 30-35% similar to mouse fibroblast growth factor receptor 3 and a kinase homologue in D. melanogaster in the tyrosine protein kinase region. STYK1/NOK was identified as being up regulated in MDA-MB-231, an estrogen receptor-alpha negative breast cancer cell line, following 12 h of estrogen treatment at 1 x 10(-9)M. On further investigation of STYK1/NOK in estrogen treated cell line MDA-MB-231, STYK1/NOK was up regulated at 6 h post treatment when compared to untreated cells. We also investigated the expression levels of STYK1/NOK in other breast cancer cell lines MCF-7, MDA-MB-231, BT-549, and MDA-MB-435S using QRT-PCR. In addition, the analysis of message accumulation was increased with other synthetic estrogen response modifiers. We propose that the regulation of STYK1/NOK is achieved independent of ER alpha and suggests further investigation to the relevance of this kinase in breast cancer progression.

• 出版日期2008-3