Methyl Jasmonate Induces ATP Biosynthesis Deficiency and Accumulation of Proteins Related to Secondary Metabolism in Catharanthus roseus (L.) G. Hairy Roots

作者：Ruiz May Eliel; De la Pena Clelia; Galaz Avalos Rosa M; Lei Zhentian; Watson Bonnie S; Sumner Lloyd W; Loyola Vargas Victor M^*

来源：Plant and Cell Physiology, 2011, 52(8): 1401-1421.

DOI：10.1093/pcp/pcr086

• Catharanthus roseus
• Hairy roots
• Methyl jasmonate
• Proteome
• Secondary metabolites

Jasmonates are specific signal molecules in plants that are involved in a diverse set of physiological and developmental processes. However, methyl jasmonate (MeJA) has been shown to have a negative effect on root growth and, so far, the biochemical mechanism for this is unknown. Using Catharanthus roseus hairy roots, we were able to observe the effect of MeJA on growth inhibition, cell disorganization and cell death of the root cap. Hairy roots treated with MeJA induced the perturbation of mitochondrial membrane integrity and a diminution in ATP biosynthesis. Furthermore, several proteins were identified that were involved in energy and secondary metabolism; the changes in accumulation of these proteins were observed with 100 mu M MeJA. In conclusion, our results suggest that a switch of the metabolic fate of hairy roots in response to MeJA could cause an increase in the accumulation of secondary metabolites. This is likely to have important consequences in the production of specific alkaloids important for the pharmaceutical industry.

• 出版日期2011-8