Optimization of Microwave-Assisted Extraction of Puerarin from Radix Puerariae using Response Surface Methodology

作者：Wu, Zhijun^*; Ruan, Hongsheng; Wang, Yanhong; Chen, Zhibao; Cui, Yudong

来源：Separation Science and Technology (Philadelphia), 2013, 48(11): 1657-1664.

DOI：10.1080/01496395.2012.752749

• microwave-assisted extraction
• plackett-burman design
• puerarin
• response surface methodology

Statistical experimentical designs were applied to optimize microwave-assisted extraction of puerarin from Radix Puerariae. The most important factors affecting the extraction procedure were determined using a Plackett-Burman design. Results indicated that the concentration of ethanol, solvent-material ratio, extraction time, and microwave power were the main factors affecting the extraction yield. These factors were further optimized using a central composite design and response surface methodology. The optimal extraction parameters were ethanol concentration of 52.36%, microwave irradiation time of 60s, microwave power of 184.8W and solvent-material ratio of 25:1(mL/g). The average experimental puerarin yield under the optimum conditions was found to be 11.97mg/g, which agreed with the predicted value of 11.8mg/g. The proposed method shows high degree of reproducibility.

• 出版日期2013-6-1
• 单位黑龙江八一农垦大学