Action of fungal beta-glucosidase on the mono-O-methylated p-nitrophenyl beta-D-glucopyranoside

作者：Nishimura Takeshi^*; Ishihara Mitsuro

来源：Holzforschung, 2009, 63(1): 47-51.

DOI：10.1515/HF.2009.004

• fungal beta-glucosidase
• p-nitrophenyl beta-D-glucopyranoside
• regio-specifically substituted glucoside
• substrate specificity

The action of p-glucosidase on the methyl derivatives of p-nitrophenyl beta-D-glucopyranoside (pNP-beta-Glc), which were regio-specifically substituted at O-2, O-3, O-4, and O-6 positions, was studied. Specifically, several beta-glucosidases isolated from brown-rot, white-rot, soft-rot fungi, and almond were investigated. These beta-glucosidases did not act on the 2, 3, and 4-O-methyl derivatives, while the 6-O-methyl one was hydrolyzed by all the enzymes to some extent. The results indicate that the methyl group at O-2, O-3, and O-4 of the glucopyranoside strongly inhibits the recognition by the beta-glucopyranoside, while the enzymes do not discriminate the structure difference between pNP-beta-Glc and its methyl derivative at O-6.

• 出版日期2009