Automatic on-chip RNA-DNA hybridization assay with integrated phase change microvalves

作者：Weng, Xuan^*; Jiang, Hai; Wang, Junsheng; Chen, Shu; Cao, Honghe; Li, Dongqing

来源：Journal of Micromechanics and Microengineering, 2012, 22(7): 075003.

DOI：10.1088/0960-1317/22/7/075003

• POLYMERASE-CHAIN-REACTION
• REAL-TIME PCR
• SALMONELLA-TYPHIMURIUM
• RAPID DETECTION
• ELISA
• FOOD
• SEQUENCE
• CULTURE
• SPP.

An RNA-DNA hybridization assay microfluidic chip integrated with electrothermally actuated phase change microvalves for detecting pathogenic bacteria is presented in this paper. In order to realize the sequential loading and washing processes required in such an assay, gravity-based pressure-driven flow and phase-change microvalves were used in the microfluidic chip. Paraffin wax was used as the phase change material in the valves and thin film heaters were used to electrothermally actuate microvalves. Light absorption measured by a photodetector to determine the concentrations of the samples. The automatic control of the complete assay was implemented by a self-coded LabVIEW program. To examine the performance of this chip, Salmonella was used as a sample pathogen. Significantly, reduction in reagent/sample consumption (up to 20 folds) was achieved by this on-chip assay, compared with using the commercial test kit following the same protocol in conventional labs. The experimental results show that the quantitative detection can be obtained in approximately 26 min, and the detection limit is as low as 10(3) CFU ml(-1). This RNA-DNA hybridization assay microfluidic chip shows an excellent potential in the development of a portable device for point-of-testing applications.

• 出版日期2012-7
• 单位大连海事大学