科研之友
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摘要
Increasing evidence suggests an association between periodontal disease and low birthweight (LBW). We previously studied the effect of lipopolysaccharide (LPS) from periodontopathogen Aggregatibacter actinomycetemcomitans (Aa) on human trophoblasts, and found that Aa-LPS induces apoptosis via the mitochondria-dependent pathway. This effect may contribute to the pathogenesis of periodontitis-associated LBW. In the present study, we observed the human placental trophoblast-like BeWo cells' response to Aa-LPS. Human placental trophoblast-like BeWo cells were cultured and treated with Aa-LPS. The time-dependant effects of Aa-LPS on cultured BeWo cells were studied using the Affymetrix GeneChipTM system (human; ca. 39500 genes). Reverse transcription-polymerase chain reaction (RT-PCR) and quantitative real-time PCR analysis of gene expression were also carried out to confirm the DNA microarray results. In BeWo cells, Aa-LPS altered many gene expressions including the alpha subunit of the heterotrimeric G protein that stimulates adenylyl cyclase (GNAS) mRNA level. The reduction of GNAS gene expression by Aa-LPS was successfully confirmed by reverse transcription-polymerase chain reaction (RT-PCR) and quantitative real-time PCR. In conclusion, since GNAS was found to be particularly important to placental and fetal growth, the reduction of GNAS gene expression by Aa-LPS may be involved in growth retardation of the fetus, and may be the molecular basis of the mechanism of periodontitis-associated LBW.
- 出版日期2013-1
- 单位天津医科大学
