Activation and selective IL-17 response of human V gamma 9V delta 2 T lymphocytes by TLR-activated plasmacytoid dendritic cells

作者：Lo Presti Elena; Caccamo Nadia; Orlando Valentina; Dieli Francesco; Meraviglia Serena^*

来源：Oncotarget, 2016, 7(38): 60896-60905.

DOI：10.18632/oncotarget.11755

• gamma delta T cells
• plasmacytoid dendritic cells
• IL-17
• TLR activation
• proliferation
• Immunology and Microbiology Section
• Immune response
• Immunity

V gamma 9V delta 2 T cells and plasmacytoid dendritic cells (pDCs) are two distinct cell types of innate immunity that participate in early phases of immune response. We investigated whether a close functional relationship exists between these two cell populations using an in vitro co-culture in a human system.
pDCs that had been activated by IL-3 and the TLR9 ligand CpG induced substantial activation of V gamma 9V delta 2 T cells upon co-culture, which was cell-to-cell contact dependent, as demonstrated in transwell experiments, but that did not involve any of the costimulatory molecules potentially expressed by pDCs or V gamma 9V2 T cells, such as ICOS-L, OX40 and CD40L. Activated pDCs selectively induced IL-17, but not IFN-gamma, responses of V gamma 9V delta 2T cells, which was dominant over the antigen-induced response, and this was associated with the expansion of memory (both central and effector memory) subsets of V gamma 9V delta 2 T cells.
Overall, our results provide a further piece of information on the complex relationship between these two populations of cells with innate immunity features during inflammatory responses.

• 出版日期2016-9-20