Development and validation of a high-performance liquid chromatography method for determination of ractopamine residue in pork samples by solid phase extraction and pre-column derivatization

作者：Ding Guanglong; Li Deguang; Qin Jiao; Zhu Juanli; Wang Baitao; Geng Qianqian; Guo Mingcheng; Punyapitak Darunee; Cao Yongsong^*

来源：Meat Science, 2015, 106: 55-60.

DOI：10.1016/j.meatsci.2015.03.016

• Ractopamine
• Residue determination
• Solid-phase extraction
• Pre-column derivatization
• High-performance liquid chromatography

Ractopamine (RAC) has been approved as a feed additive for swine, cattle or turkey, and is likely to have residue in edible animal products and may pose a potential risk for consumer health. Therefore, it is essential to establish a method to detect the residue of RAC in animal products. This work presents a rapid and sensitive HPLC method for the determination of RAC in pork samples with pre-column derivatization. The RAC derivative was separated on a kromasil C18 column and detected at 284 nm with a UV detector. The detection capability (CC beta) was 0.078 mu g g(-1) and the linearity was established over the concentration range of 0.15-100.0 mu g g(-1). The overall mean recovery in spike range of 0.2 mu g g(-1) to 100 mu g g(-1) was 89.9% with the overall mean relative standard deviation of 4.1%. This method can be used for the quantification of RAC in pork samples and help to establish adequate monitoring of the residue of RAC.

• 出版日期2015-8
• 单位中国农业大学