Direct polymerase chain reaction (PCR) from human whole blood and filter-paper-dried blood by using a PCR buffer with a higher pH

作者：Bu Ying; Huang Huan; Zhou Guohua^*

来源：Analytical Biochemistry, 2008, 375(2): 370-372.

DOI：10.1016/j.ab.2008.01.010

We described a novel approach to directly amplify genomic DNA from whole blood and dried blood spotted on filter paper without any DNA isolation by using the PCR buffer with a higher pH, which was optimized as pH 9.1-9.6. Direct PCR on blood treated with various anticoagulants showed that the buffer worked well with the blood treated by citrate, EDTA, or heparinate. DNA fragments with different lengths could be efficiently amplified directly from various forms of blood samples.

• 出版日期2008-4-15
• 单位中国药科大学; 南京大学; 河北医科大学