A novel FRET-based fluorescent chemosensor of beta-cyclodextrin derivative for TNT detection in aqueous solution

作者：Feng, Lijuan; Tong, Cuiyan^*; He, Yao; Liu, Bingxin; Wang, Chunyu; Sha, Jie; Lu, Changli

来源：Journal of Luminescence, 2014, 146: 502-507.

DOI：10.1016/j.jlumin.2013.10.039

• beta-Cyclodextrin
• FITC
• FRET
• Chemosensor
• TNT
• Aqueous solution

A new fluorescent sensor based on single water-soluble beta-cyclodextrin (beta-CD) molecule as the support carrier of donor and acceptor complex was designed, and used for TNT detection by fluorescence resonance energy transfer (FRET). In this sensing platform, per-6-amino-beta-CD (per-6-NH2-beta-CD) was used as the detection vehicle. The probe dye of fluorescein 5(6)-isothiocyanate (FITC) was covalently linked onto the per-6-NH2-beta-CD rim with the grafting molar ratio of 1:1, the residual amino groups of per-6-NH2-beta-CD can adsorb TNT molecules by forming Meisenheimer complex (TNT-amine complex) with TNT. The absorbtion spectrum of this complex has a spectral overlapping with the emission of FITC in aqueous solution, so it can strongly suppress the fluorescence emission of the adjacent FITC through FRET on beta-CD vehicle. This FRET-based fluorescent sensing technique provides a facile, ultrasensitive and selective detection method for TNT molecule. The observed litnear fluorescence intensity change could allow the quantitative detection TNT with the deection limit of 20 nM in water.

• 出版日期2014-2
• 单位东北师范大学; 北华大学