A PRACTICAL METHODOLOGY FOR IgG PURIFICATION VIA CHITOSAN BASED MAGNETIC NANOPARTICLES

作者：Kavaz Doga; Odabas Sedat; Denkbas Emir Baki; Vaseashta Ashok^*

来源：Digest Journal of Nanomaterials and Biostructures, 2012, 7(3): 1165-1177.

• Chitosan
• Magnetic Nanoparticles
• IgG
• separation

The objective of this investigation is to develop a simple, rapid, and practical separation system for IgG separation using magnetic chitosan nanoparticles. Magnetic nanoparticles were prepared by co-precipitation method and coated with a biocompatible polymer chitosan by varying molecular weights. Morphological analyses of magnetic chitosan nanoparticles were investigated by Scanning Electron Microscope (SEM) and Zeta-Sizer. Chemical structure of the nanoparticles was analyzed by Fourier Transform Infra-Red Spectroscopy (FTIR) and magnetic properties were investigated using Electron Spin Resonance (ESR) and Vibrating Sample Magnetometer (VSM). For the purification of IgG, a well-known protein Concanavalin A (Con-A), having a strong affinity to the IgG molecules, was immobilized onto magnetic chitosan nanoparticles. IgG purification was performed using a batch separation system made locally. It was observed that IgG can easily be purified from fresh human plasma with high efficacy, using a simple and inexpensive system prepared locally. Nanoparticle size, IgG concentrations and the Con-A concentrations were investigated to get an optimum separation efficiency.

• 出版日期2012-9