Association of TNF-alpha (-238 and-308) promoter polymorphisms with susceptibility of oral squamous cell carcinoma in North Indian population

作者：Singh Prithvi Kumar; Bogra Jaishri; Chandra Girish^*; Ahmad Mohammad Kaleem; Gupta Rajni; Kumar Vijay; Jain Amita; Mahdi Abbas Ali

来源：Cancer Biomarkers, 2015, 15(2): 125-131.

DOI：10.3233/CBM-140444

• Oral squamous cell carcinoma
• cytokines
• tumor necrosis factor-alpha
• inflammation
• genotype

BACKGROUND: The pro-inflammatory cytokines play an essential role in immune response and are involved in a variety of inflammatory and infectious disease. Tumor necrosis factor alpha (TNF-alpha) gene polymorphism has been a potential determinant of susceptibility to various types of cancer.
OBJECTIVE: To evaluate the association of TNF-alpha gene promoter (-238) G/A and (-308) G/A polymorphisms with the susceptibility of OSCC patients in North Indian population.
METHODS: A total 272 patients with OSCC and 185 healthy volunteers were genotypes for the TNF-alpha (-238) G/A and (-308) G/A gene polymorphism. Genotypes were identified by polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP). Genotype frequencies were evaluated by Chi-square test and Odds ratio (OR) relative risk.
RESULTS: TNF-alpha (-238) G/A polymorphism was significantly associated with OSCC patients as compared to healthy volunteers (GG vs. GA: OR = 0.3500, 95% CI = 0.1289-09502; p = 0.036; G vs. A: OR = 0.3589 1.477, 95% CI = 0.1335-0.9652; p = 0.0386). No significant association was found in TNF-alpha (-308) G/A gene polymorphism with OSCC patients and controls.
CONCLUSIONS: We conclude that the TNF-alpha (-238) G/A polymorphism was significantly associated with OSCC however TNF-alpha (-308) G/A polymorphism was not associated in OSCC patients.

• 出版日期2015