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摘要
In this study, the antibacterial activity and mechanism of berberine against avian Pasteurella multocida were investigated by evaluating bacteria growth, observing ultrastructure changes and studying the synthesis of protein and DNA. The antibacterial susceptibility test indicated the minimum inhibition concentration (MIC) of berberine against P. multocida was 39 mu g/mL and the antibacterial kinetic curves showed that the antibacterial activity of berberine was in a concentration-time-dependent manner. After treatment with berberine (39 mu g/mL), the bacteria cells were severely damaged, irregular cell shape, ruptured cell wall and membrane, condensed cytoplasm or lost cytoplasm were observed under the transmission electron microscopy. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and 2-(4-amidinophenyl)-1H-indole-6-carboxamidine (DAPI) stain assays demonstrated that berberine could inhibit the synthesis of protein and DNA. In conclusion, these results suggested that berberine inhibit the cell function of P. multocida by damaging the cell structure and inhibiting synthesis of protein and DNA, resulting in the cell death.
- 出版日期2016
- 单位四川农业大学; 宜宾学院
