An insight into the influence of low dose irradiation pretreatment on the microbial decolouration and degradation of Reactive Red-120 dye

作者：Paul Jhimli^*; Kadam Avinash A; Govindwar Sanjay P; Kumar Pranaw; Varshney Lalit

来源：Chemosphere, 2013, 90(4): 1348-1358.

DOI：10.1016/j.chemosphere.2012.07.049

• Reactive azo dyes
• Radiation
• Biodegradation
• Toxicity
• ESI-MS
• GC-MS

The influence of low dose irradiation pretreatment on the microbial decolouration and degradation of Reactive Red-120 (RR-120) dye was investigated in detail by using Pseudomonas sp. SUK1. About 27%, 56% and 66% decolouration of 150 ppm RR-120 dye solution was observed by applying 0, 0.5 and 1 kGy doses, respectively, in the first step followed by microbial treatment for 24 h under static condition. Similarly, about 70%, 88% and 90% TOC removal was observed by applying 0, 0.5 and I kGy doses, respectively, in the first step followed by the microbial treatment for 96 h under static condition. The radiation induced fragmented products of RR-120 at doses of 0.5 and 1 kGy were investigated by FUR and electrospray ionization-MS analysis. The induction of the enzymes viz. laccase, tyrosinase, azoreductase and NADH-2,6-dichlorophenol indophenol reductase was studied in the decolourised solution obtained after irradiating 150 ppm RR-120 dye solution with 0 and 1 kGy doses followed by the microbial treatment for 96 h under static condition. The enzymatic degradation products were studied by FTIR, HPLC and GC-MS. The toxicity study of the treated dye solution on plants revealed the degradation of RR-120 into non-toxic products by combined radiation-microbial treatment. This study explores a reliable and promising way to use industrially viable dose (<= 1 kGy) and microbial strain viz. Pseudomonas sp. SUK1 for permissible safe disposal of dye solutions from textile industries.

• 出版日期2013-1