The Distinct Functional Roles of the Inner and Outer Chloroplast Envelope of Pea (Pisum sativum) As Revealed by Proteomic Approaches

作者：Gutierrez Carbonell Elain; Takahashi Daisuke; Lattanzio Giuseppe; Rodriguez Celma Jorge; Kehr Julia; Soll Juergen; Philippar Katrin; Uemura Matsuo; Abadia Javier; Flor Lopez Millan Ana

来源：Journal of Proteome Research, 2014, 13(6): 2941-2953.

DOI：10.1021/pr500106s

• 2-DE electrophoresis
• chloroplast envelope
• database
• membrane proteins
• pea
• Pisum sativum
• shotgun proteomics

Protein profiles of inner (IE) and outer (OE) chloroplast envelope membrane preparations from pea were studied using shotgun nLC-MS/MS and two-dimensional electrophoresis, and 589 protein species (NCBI entries) were identified. The relative enrichment of each protein in the IE/OE pair of membranes was used to provide an integrated picture of the chloroplast envelope. From the 546 proteins identified with shotgun, 321 showed a significant differential distribution, with 180 being enriched in IE and 141 in OE. To avoid redundancy and facilitate in silica localization, Arabidopsis homologues were used to obtain a nonredundant list of 409 envelope proteins, with many showing significant OE or IE enrichment. Functional classification reveals that IE is a selective barrier for transport of many metabolites and plays a major role in controlling protein homeostasis, whereas proteins in OE are more heterogeneous and participate in a wide range of processes. Data support that metabolic processes previously described to occur in the envelope such as chlorophyll and tocopherol biosynthesis can be ascribed to the IE, whereas others such as carotenoid or lipid biosynthesis occur in both membranes. Furthermore, results allow empirical assignation to the IE and/or OE of many proteins previously assigned to the bulk chloroplast envelope proteome.

• 出版日期2014-6