New Mutations in Chronic Lymphocytic Leukemia Identified by Target Enrichment and Deep Sequencing

作者：Domenech Elena^*; Gomez Lopez Gonzalo; Gzlez Pena Daniel; Lopez Mar; Herreros Beatriz; Menezes Juliane; Gomez Lozano Natalia; Carro Angel; Grana Osvaldo; Pisano David G; Dominguez Orlando; Garcia Marco Jose A; Piris Miguel A; Sanchez Beato Margarita

来源：PLos One, 2012, 7(6): e38158.

DOI：10.1371/journal.pone.0038158

Chronic lymphocytic leukemia (CLL) is a heterogeneous disease without a well-defined genetic alteration responsible for the onset of the disease. Several lines of evidence coincide in identifying stimulatory and growth signals delivered by B-cell receptor (BCR), and co-receptors together with NFkB pathway, as being the driving force in B-cell survival in CLL. However, the molecular mechanism responsible for this activation has not been identified. Based on the hypothesis that BCR activation may depend on somatic mutations of the BCR and related pathways we have performed a complete mutational screening of 301 selected genes associated with BCR signaling and related pathways using massive parallel sequencing technology in 10 CLL cases. Four mutated genes in coding regions (KRAS, SMARCA2, NFKBIE and PRKD3) have been confirmed by capillary sequencing. In conclusion, this study identifies new genes mutated in CLL, all of them in cases with progressive disease, and demonstrates that next-generation sequencing technologies applied to selected genes or pathways of interest are powerful tools for identifying novel mutational changes.

• 出版日期2012-6-1