Phytochemistry and Preliminary Assessment of the Antibacterial Activity of Chloroform Extract of Amburana cearensis (Allemao) AC Sm. against Klebsiella pneumoniae Carbapenemase-Producing Strains

作者：Sa Mirivaldo Barros; Ralph Maria Taciana; Oliveira Nascimento Danielle Cristina; Ramos Clecio Souza; Serafin Barbosa Isvania Maria; Sa Fabricio Bezerra; Lima Filho J V

来源：Evidence-Based Complementary and Alternative Medicine, 2014, 2014: 786586.

DOI：10.1155/2014/786586

The chloroform extract of the stem bark of Amburana cearensis was chemically characterized and tested for antibacterial activity. The extract was analyzed by gas chromatography and mass spectrometry. The main compounds identified were 4-methoxy-3-methylphenol (76.7%), triciclene (3.9%), alpha-pinene (1.0%), beta-pinene (2.2%), and 4-hydroxybenzoic acid (3.1%). Preliminary antibacterial tests were carried out against species of distinct morphophysiological characteristics: Escherichia coli, Salmonella enterica Serotype Typhimurium, Pseudomonas aeruginosa, Staphylococcus aureus, Listeria monocytogenes, and Bacillus cereus. The minimum inhibitory concentration (MIC) was determinate in 96-well microplates for the chloroform extract and an analogue of the main compound identified, which was purchased commercially. We have shown that plant's extract was only inhibitory (but not bactericidal) at the maximum concentration of 6900 mu g/mL against Pseudomonas aeruginosa and Bacillus cereus. Conversely, the analogue 2-methoxy-4-methylphenol produced MICs ranging from 215 to 431 mu g/mL against all bacterial species. New antibacterial assays conducted with such chemical compound against Klebsiella pneumoniae carbapenemase-producing strains have shown similar MIC results and minimum bactericidal concentration (MBC) of 431 mu g/mL. We conclude that A. cearensis is a good source of methoxy-methylphenol compounds, which could be screened for antibacterial activity against multiresistant bacteria from different species

• 出版日期2014