An Electrochemical Method for Sensitive Determination of Antioxidant Capacity

作者：Ying Wang; Calas Blanchard Carole^*; Cortina Puig Montserrat; Liu Baohong; Marty Jean Louis

来源：Electroanalysis, 2009, 21(12): 1395-1400.

DOI：10.1002/elan.200804537

• Antioxidant capacity
• Hydroxyl radicals
• Electrochemical detection
• 4-Hydroxybenzoic acid
• TiO(2) nanoparticles

A simple and sensitive electrochemical method for the determination of antioxidant capacity has been Studied. 4-hydroxybenzoic acid (4-HBA) was used as a trapping agent for photogenerated (center dot)OH radicals, leading to 3,4-dihydroxybenzoic acid (3,4-DHBA). Square-wave voltammetry was used to quantify the amount of 3,4-DHBA formed from the reaction between 4-HBA and (center dot)OH. Addition of antioxidants induced the competition between 4-HBA and antioxidants toward (center dot)OH elimination, resulting in a decrease of the measured current. The IC(50) for different standard antioxidants was calculated and expressed in Trolox equivalent antioxidant capacity (TEAC). The proposed method was successfully compared to a fluorimetric one.

• 出版日期2009-6
• 单位复旦大学