Mix-and-diffuse serial synchrotron crystallography

作者：Beyerlein Kenneth R; Dierksmeyer Dennis; Mariani Valerio; Kuhn Manuela; Sarrou Iosifina; Ottaviano Angelica; Awel Salah; Knoska Juraj; Fuglerud Silje; Jonsson Olof; Stern Stephan; Wiedorn Max O; Yefanov Oleksandr; Adriano Luigi; Bean Richard; Burkhardt Anja; Fischer Pontus; Heymann Michael; Horke Daniel A; Jungnickel Katharina E J; Kovaleva Elena; Lorbeer Olga; Metz Markus; Meyer Jan; Morgan Andrew; Pande Kanupriya; Panneerselvam Saravanan; Seuring Carolin

来源：IUCrJ, 2017, 4(6): 769-777.

DOI：10.1107/S2052252517013124

• drug discovery
• protein structure
• X-ray crystallography
• serial crystallography
• time-resolved studies
• lysozyme

Unravelling the interaction of biological macromolecules with ligands and substrates at high spatial and temporal resolution remains a major challenge in structural biology. The development of serial crystallography methods at X-ray free-electron lasers and subsequently at synchrotron light sources allows new approaches to tackle this challenge. Here, a new polyimide tape drive designed for mix-and-diffuse serial crystallography experiments is reported. The structure of lysozyme bound by the competitive inhibitor chitotriose was determined using this device in combination with microfluidic mixers. The electron densities obtained from mixing times of 2 and 50 s show clear binding of chitotriose to the enzyme at a high level of detail. The success of this approach shows the potential for high-throughput drug screening and even structural enzymology on short timescales at bright synchrotron light sources.

• 出版日期2017-11