Nanoceria and bulk cerium oxide effects on the germination of Asplenium adiantum-nigrum spores

作者：Gomez Garay Aranzazu^*; Pintos Beatriz; Manzanera Jose Antonio; Prada Carmen; Martin Luisa; Gabriel y Galan Jose Maria

来源：Forest Systems, 2016, 25(3): UNSP e067.

DOI：10.5424/fs/2016253-09294

• Nanoparticles
• rhizoid
• prothallus
• chloroplast
• fern

Aim of the study: The effect of cerium oxide engineered nanoparticles on the spore germination of the fern Asplenium adiantum-nigrum.
Area of study: France, Britanny Region, Finistere Department, Plougonvelin, in rocks near the sea.
Material and methods: Asplenium spores were cultured in vitro on agar medium with Nano-CeO2 (less than 25 nm particle size) and bulk-CeO2. The addition of each nano-and bulk particles ranged from 0 to 3000 mg L-1. Observations on rhizoidal and prothallial cells during first stages of gametophyte development were made. The No-Observed-Adverse-Effect concentration (NOAEC) and Lowest-Observed-Adverse-Effect-Concentration (LOEC) values for spore germination rate data were analyzed.
Main results: Germination was speeded up by 100 to 2000 mg L-1 nanoceria, while bulk cerium oxide had the same effect for 500 to 2000 mg L-1 concentrations. Present results showed cellular damage in the protonema while rhizoid cells seemed not to be affected, as growth and membrane integrity remained.
Research highlights: Both nanosized and bulk cerium oxide are toxic for the fern Asplenium adiantum-nigrum, although diverse toxicity patterns were shown for both materials. Diverse toxic effects have been observed: chloroplast membrane damage and lysis, cell wall and membrane disruption which leads to cell lysis; and alterations in morphology and development.

• 出版日期2016