A new assignment technique of 2D-NMR spectra by spin-lock sequence to a tripeptide containing tryptophan in water

作者：Watanabe Eiji^*; Yamakura Fumiyuki; Kan Hirosaki

来源：Magnetic Resonance in Chemistry, 2010, 48(3): 205-209.

DOI：10.1002/mrc.2567

• NMR
• (1)H
• TORO
• TOROTO
• PFG
• tryptophan
• indole

We developed a new assignment technique of tryptophan residues using pulsed field gradient TOCSY-ROESY (PFG-TORO) and pulsed field gradient TOCSY-ROESY-TOCSY (PFG-TOROTO) techniques in water. Connectivity from beta H to zeta 2H (H-7) via epsilon 1H (H-1) and delta 1H (H-2) in the TORO spectrum and from beta H to zeta 3H (H-5) and eta 2H (H-6) via epsilon 1H (H-1) and delta 1H (H-2) in the TOROTO spectrum could be able to assign each of the protons of the indole rings.

• 出版日期2010-3