Neurotrophin-3 Regulates Synapse Development by Modulating TrkC-PTP sigma Synaptic Adhesion and Intracellular Signaling Pathways

作者：Han Kyung Ah; Woo Doyeon; Kim Seungjoon; Choii Gayoung; Jeon Sangmin; Won Seoung Youn; Kim Ho Min; Do Heo Won; Um Ji Won^*; Ko Jaewon^*

来源：Journal of Neuroscience, 2016, 36(17): 4816-4831.

DOI：10.1523/JNEUROSCI.4024-15.2016

• excitatory synapse
• neurotrophin-3
• PTP sigma
• synaptic cell adhesion
• TrkC

Neurotrophin-3 (NT-3) is a secreted neurotrophic factor that binds neurotrophin receptor tyrosine kinase C (TrkC), which in turn binds to presynaptic protein tyrosine phosphatase sigma (PTP sigma) to govern excitatory synapse development. However, whether and how NT-3 cooperates with the TrkC-PTP sigma synaptic adhesion pathway and TrkC-mediated intracellular signaling pathways in rat cultured neurons has remained unclear. Here, we report that NT-3 enhances TrkC binding affinity for PTP sigma. Strikingly, NT-3 treatment bidirectionally regulates the synaptogenic activity of TrkC: at concentrations of 10-25 ng/ml, NT-3 further enhanced the increase in synapse density induced by TrkC overexpression, whereas at higher concentrations, NT-3 abrogated TrkC-induced increases in synapse density. Semi-quantitative immunoblotting and optogenetics-based imaging showed that 25 ng/ml NT-3 or light stimulation at a power that produced a comparable level of NT-3 (6.25 mu W) activated only extracellular signal-regulated kinase (ERK) and Akt, whereas 100 ng/ml NT-3 (light intensity, 25 mu W) further triggered the activation of phospholipase C-gamma 1 and CREB independently of PTP sigma. Notably, disruption of TrkC intracellular signaling pathways, extracellular ligand binding, or kinase activity by point mutations compromised TrkC-induced increases in synapse density. Furthermore, only sparse, but not global, TrkC knock-down in cultured rat neurons significantly decreased synapse density, suggesting that intercellular differences in TrkC expression level are critical for its synapse-promoting action. Together, our data demonstrate that NT-3 is a key factor in excitatory synapse development that may direct higher-order assembly of the TrkC/PTP sigma complex and activate distinct intracellular signaling cascades in a concentration-dependent manner to promote competition-based synapse development processes.

• 出版日期2016-4-27