A label-free method for studying DNA sequence recognition of mitoxantrone based on resonance light-scattering technique

作者：Chen, Zhanguang^*; Zhang, Guomin; Chen, Xi; Chen, Junhui; Liu, Jinbin

来源：Journal of Antibiotics, 2012, 65(10): 517-522.

DOI：10.1038/ja.2012.58

• anticancer drugs
• mitoxantrone
• oligonucleotides
• recognition
• resonance light scattering
• selectivity

A label-free method for studying DNA sequence recognition of mitoxantrone (MIT) by resonance light-scattering (RLS) technique has been developed in this contribution. Through the RLS spectra, the selective no-covalent interactions between MIT and double-stranded DNA, single-stranded DNA (ssDNA), oligonucleotides were systematically studied. The results of the experiments displayed that MIT had an obvious preference to ssDNA with the K (K-RLS), 15.16 mmol mg(-1) and the number of binding sites (N), 8.68 x 10(-4) mmol mg(-1). Besides, it was found that MIT had a preference to sequences that were rich in guanine and cytosine bases with K-RLS, 17.29 l mmol(-1) and N, 1.19 x 10(-2) l mmol(-1). The recognition mechanisms were well discussed and by fluorescence method and atom force microscopy, the RLS technique was confirmed to be a reliable method in this study. Compared with other methods, what the RLS strategy displayed was the direct interaction between anticancer drugs and DNA in vitro without the influence of a foreign substance. Thus, it can be a simple, fast and label-free strategy for DNA sequence recognition studies of DNA-targeted anticancer drugs. The Journal of Antibiotics (2012) 65, 517-522; doi:10.1038/ja.2012.58; published online 18 July 2012

• 出版日期2012-10
• 单位广东药科大学; 汕头大学; 北京大学