A simple and small-scale sample preparation technique to determine canthaxanthin in hen egg yolk

作者：Furusawa Naoto^*

来源：Food Chemistry, 2011, 124(4): 1643-1646.

DOI：10.1016/j.foodchem.2010.08.017

• Canthaxanthin
• Hen egg yolk
• Solid-phase extraction mini-cartridge
• HPLC

This paper describes an easy, small-scale method of sample preparation followed by a reversed-phase (RP) high-performance liquid chromatography (HPLC) for quantifying canthaxanthin (CX) in egg yolk. The egg yolk extract including CX was purified by using a normal-phase InterSep (R)-CN mini-syringe-column as a solid-phase extraction column. HPLC was performed on a C8 column with an isocratic mobile phase and diode array detector. The proposed method was validated by the analyses of spiked egg yolk samples; resulting recoveries (>= 94.5%; RSD <= 2.8%), analytical total time (<1 h/sample), and limit of quantitation (0.5 mu g/g). The decision limit and detection capability were 25.04 and 25.07 mu g/g, respectively.

• 出版日期2011-2-15