Antiviral Action of Methylated beta-Lactoglobulin on the Human Influenza Virus A Subtype H3N2

作者：Sitohy Mahmoud; Besse Bernard; Billaudel Sylviane; Haertle Thomas; Chobert Jean Marc^*

来源：Probiotics and Antimicrobial Proteins, 2010, 2(2): 104-111.

DOI：10.1007/s12602-010-9036-5

• Beta-lactoglobulin
• Esterification
• H3N2
• Antiviral activity

Antiviral activity of methylated beta-lactoglobulin (Met-BLG) against H3N2 infected into MDCK cell lines depended on concentration of Met-BLG, viral load, and duration of infection. IC50% of the hemagglutination activity for 1 and 0.2 MOI (multiplicity of infection) after 24 h of incubation at 37 degrees C in the presence of 5% CO2 were 20 +/- 0.8 and 17 +/- 0.7 mu g mL(-1) Met-BLG, respectively. Longer incubation period (4 days) was associated with low IC50% of the hemagglutination activity (7.1 +/- 0.3 mu g mL(-1) Met-BLG) and low IC50% of immunofluorescence of viral nucleoproteins (9.7 +/- 0.4 mu g mL(-1) Met-BLG) when using 0.2 and 0.1 MOI, respectively. A concentration of 25 mu g mL(-1) of Met-BLG reduced the amount of replicating virus by about 2 and 1.3 logs when the viral load was 0.01 and 0.1 MOI, respectively, while higher concentrations reduced it by about 5-6 logs. Antiviral action of Met-BLG was coupled with a cellular protective action, which reached 100% when using 0.01 and 0.1 MOI and 83% when using 1.0 MOI. The time of MetBLG addition after the viral infection was determinant for its antiviral efficacy and for its protection of the infected MDCK cell lines. Anti-hemagglutination action and cell protective action decreased gradually and in parallel with the delay in the time of Met-BLG addition to disappear totally after 10 h delay.

• 出版日期2010-6