Microbial degradation of endosulfan in contaminated soil with the elution of surfactants

作者：Deng, Fei; Xiong, Bailian; Chen, Benshou; Zheng, Guocan; Zhang, Jinzhong^*

来源：Environmental Science and Pollution Research, 2016, 23(13): 13268-13275.

DOI：10.1007/s11356-016-6523-6

• Endosulfan
• Microbial degradation
• Soil
• Surfactant
• Elution

In this work, an endosulfan-degrading strain was isolated from the aged soil contaminated by endosulfan, and identified as Ochrobactrum sp. EB-4 by 16S rDNA sequence analysis. The microbial degradation characteristics of endosulfan in three eluents (Tween 80 + SDS, Tween 80 + Na2SiO3, Tween 80 + SDS + Na2SiO3) were investigated. The results showed that the degradation percents of alpha-, beta-endosulfan in the three eluents were 86.83 %similar to 92.91 % and 88.90 %similar to 93.94 % in 15 days, respectively. The degradation process can be well described by the first-order kinetic model, and the half-times of alpha-endosulfan in eluent 1 similar to eluent 3 were 3.83, 5.29, and 4.53 days, while those of beta-endosulfan were 3.35, 4.50 and 3.79 days, respectively. The endosulfan diol and endosulfan sulfate as main metabolites were detected, and the former can be further degraded by this strain, which revealed that the simultaneously happened hydrolysis and oxidation reactions were the main degradation processes, and dominated by hydrolysis reaction. After 5 days of washing with the eluents, 56.00 similar to 84.33 % of alpha-endosulfan, and 46.49 similar to 68.56 % of beta-endosulfan in soil were eluted, respectively, and can be entirely biodegraded in 12 days, which indicated that the microbial degradation was the rate-determining step.

• 出版日期2016-7
• 单位遵义师范学院; 西南大学