Automated and portable solid phase extraction platform for immuno-detection of 17 beta-estradiol in water

作者：Heub Sarah^*; Tscharner Noe; Monnier Veronique; Kehl Florian; Dittrich Petra S; Follonier Stephane; Barbe Laurent

来源：Journal of Chromatography A, 2015, 1381: 22-28.

DOI：10.1016/j.chroma.2014.12.076

• Sample preparation
• Solid phase extraction
• Water analysis
• 17 beta-Estradiol
• Enzyme-linked immuno-sorbent assay

A fully automated and portable system for solid phase extraction (SPE) has been developed for the analysis of the natural hormone 17 beta-estradiol (E2) in environmental water by enzyme linked immuno-sorbent assay (ELISA). The system has been validated with de-ionized and artificial sea water as model samples and allowed for pre-concentration of E2 at levels of 1, 10 and 100 ng/L with only 100 ml of sample. Recoveries ranged from 24 +/- 3% to 107 +/- 6% depending on the concentration and sample matrix. The method successfully allowed us to determine the concentration of two seawater samples. A concentration of 15.1 +/- 0.3 ng/L of E2 was measured in a sample obtained from a food production process, and 8.8 +/- 0.7 ng/L in a sample from the Adriatic Sea. The system would be suitable for continuous monitoring of water quality as it is user friendly, and as the method is reproducible and totally compatible with the analysis of water sample by simple immunoassays and other detection methods such as biosensors.

• 出版日期2015-2-13