Multiscale measurement of cardiac energetics

作者:Goo Soyeon; Toan Pham; Han Jun Chiew; Nielsen Poul; Taberner Andrew; Hickey Anthony; Loiselle Denis*
来源:CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, 2013, 40(9): 671-681.
DOI:10.1111/1440-1681.12139

摘要

1. Herein we describe our laboratories%26apos; experimental methods for interrogating cardiac energetics at the organ (whole heart), tissue (trabecula) and perforated fibre (mitochondrial) levels. 2. In whole heart and trabecula experiments, we focus on measuring pressure-volume (force-length) work and oxygen consumption (heat production) from which mechanical efficiency is derived. In both preparations (i. e. across scales differing by three orders of magnitude) we find efficiency values of 10%-15%. 3. Mitochondrial experiments invoke a trio of titration protocols to yield information on oxygen consumption, ATP flux, membrane potential, electron leak and reactive oxygen species production, the latter two of which index energy transfer inefficiencies.

  • 出版日期2013-9