Development of a new gquadruplex biosensor with the functionalized SBA-15-Au nanoparticles: A platform for selecting gquadruplex-binding ligands

作者：Bagheryan Zahra; Raoof' Jahan Bakhsh^*; Ojani Reza

来源：Sensors and Actuators B: Chemical , 2015, 213: 124-130.

DOI：10.1016/j.snb.2015.02.071

• Gquadruplex DNA
• Electrochemical biosensor
• Au nanoparticles
• Mesoporous structure
• Telomere

To investigate the Gquadruplex DNA (G(4)DNA) structure, the electrochemical biosensor was prepared by modifying the screen-printed graphite electrode (SPE) with the synthesized SBA-N-propylpipyrazine-N-(2-mercaptopropane-1-one) (SBA@NPPNSH) mesoporous structures and Au nanoparticles (AuNPs). The pores of SBA@NPPNSH structures can act as a good platform for the preconcentration of AuNPs. The SH organic groups inside these pores help to increase the electrodeposition of the AuNPs on the surface. Then SH-G(4)DNA was linked to the modified electrode by an AuNPs-S bond. This platform was used to study the G(4)DNA/drug interaction. The morphology of the modified electrode was characterized by the scanning electron microscope (SEM). The interaction of different ligands, such as Cephalexine (CEF), with G(4)DNA was studied in a Tris-HCl buffer as well as in the presence of [Fe(CN)(6)](3-), a redox label, using the CV method. The CV current decreased in response to increasing the concentration of drug due to the G(4)DNA/drug interaction. The G-quadruplex formation and the interaction mechanism between G(4)DNA and the drug were also identified by the circular dichroism (CD) measurements. Studying the selectivity was performed using different double-stranded (ds) DNA sequences.

• 出版日期2015-7-5