科研之友
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摘要
BACKGROUNDVisible-near infrared spectroscopy remains a method of increasing interest as a fast alternative for the evaluation of fruit quality. The success of the method is assumed to be achieved by using large sets of samples to produce robust calibration models. In this study we used representative samples of an early and a late season apple cultivar to evaluate model robustness (in terms of prediction ability and error) on the soluble solids content (SSC) and acidity prediction, in the wavelength range 400-1100nm. %26lt;br%26gt;RESULTSA total of 196 middle-early season and 219 late season apples (Malus domestica Borkh.) cvs Aroma%26apos; and Holsteiner Cox%26apos; samples were used to construct spectral models for SSC and acidity. Partial least squares (PLS), ridge regression (RR) and elastic net (EN) models were used to build prediction models. Furthermore, we compared three sub-sample arrangements for forming training and test sets (smooth fractionator%26apos;, by date of measurement after harvest and random). Using the smooth fractionator%26apos; sampling method, fewer spectral bands (26) and elastic net resulted in improved performance for SSC models of Aroma%26apos; apples, with a coefficient of variation CVSSC = 13%. The model showed consistently low errors and bias (PLS/EN: R-cal(2)=0.60/0.60; SEC = 0.88/0.88 degrees Brix; Bias(cal)=0.00/0.00; R-val(2)=0.33/0.44; SEP = 1.14/1.03; Bias(val)=0.04/0.03). However, the prediction acidity and for SSC (CV = 5%) of the late cultivar Holsteiner Cox%26apos; produced inferior results as compared with Aroma%26apos;. %26lt;br%26gt;CONCLUSIONIt was possible to construct local SSC and acidity calibration models for early season apple cultivars with CVs of SSC and acidity around 10%. The overall model performance of these data sets also depend on the proper selection of training and test sets. The smooth fractionator%26apos; protocol provided an objective method for obtaining training and test sets that capture the existing variability of the fruit samples for construction of visible-NIR prediction models. The implication is that by using such efficient%26apos; sampling methods for obtaining an initial sample of fruit that represents the variability of the population and for sub-sampling to form training and test sets it should be possible to use relatively small sample sizes to develop spectral predictions of fruit quality. Using feature selection and elastic net appears to improve the SSC model performance in terms of R-2, RMSECV and RMSEP for Aroma%26apos; apples.
- 出版日期2013-12
