Mitochondria-regulated death pathway mediates (DIPP-L-Leu)(2)-L-LysOCH(3)-induced K562 cells apoptosis.

作者：Yang, J; Jiang, YY^*; Liu, F; Zhao, YF

来源：Protein and Peptide Letters, 2006, 13(2): 129-134.

DOI：10.2174/092986606775101643

• phosphoryl dipeptide
• apoptosis
• caspase
• mitochondria

(DIPP-L-Leu)(2)-L-LysOCH(3) is a diisopropylphosphoryl dipeptide which is known to induce apoptosis of human leukemia K562 cells. The molecular and cellular mechanisms involved in this process remain to be clarified. Herein, we show that (DIPP-L-Leu)(2)-L-LysOCH(3)-induced apoptosis is associated with cytosolic accumulation of cytochrome c, sustained loss of mitochondrial transmembrane potential (MMP), transient generation of reactive oxygen species (ROS) and elevation of intracellular Ca2 concentration. A specific caspase assay reveals an increase in caspase-9 and caspase-3 activity but no change in caspase-8 activity. Immunofluorescence analysis indicates that (DIPP-L-Leu)2-L-LysOCH3 induced upregulation of pro-apoptotic Bax and downregulation of anti-apoptotic Bcl-2 and Bcl-x(L). These results suggest that the mitochondria-regulated death pathway mediates (DIPP-L-Leu)(2)-L-LysOCH(3)-induced K562 cells apoptosis.

• 出版日期2006
• 单位清华大学