Analysis of Agaro-oligosaccharide by Liquid Chromatography Coupled with Electrospray Ionization-Quadrupole-Time of Flight-Mass Spectrometry

作者：Xu Yan Ting; Wang Xiu Juan; Su Xiao Ling; Xu Ji Lin; Chen Hai Min; Chen Juan Juan; Yan Xiao Jun^*

来源：Chinese Journal of Analytical Chemistry, 2011, 39(12): 1798-1804.

DOI：10.3724/SP.J.1096.2011.01798

• Agaro-oligosaccharides
• Amide chromatogram column
• Liquid chromatography
• Electrospray ionization-quadrupole-time of flight-mass spectrometry

A method was developed to isolate and identify agaro-oligosaccharides by ultra performance liquid chromatography-electrospray ionization-quadrupole-time of flight mass spectrometry (UPLC-ESI-Q-TOF-MS). In the experiment, three chromatographic columns (BEH Amide, BEH C, and Atlantis T3) were used to isolate agaro-oligosacchrides. It was found that the BEH Amide had excellent isolation effect. Agarobiose oligomers with degree of polymerization (DP) ranging from 3 to 29 could be isolated rapidly and sensitively, and no derivatization procedure was needed. However, after derived with alpha-naphthylamine, agarobiose oligomers could be separated better by Atlantis T3 column, and a perfect AU spectrum was obtained. When combined liquid chromatography with ESI-Q-TOF-MS, accurate structure information of each chromatogram peak was obtained, the spectra were clear and the molecular weight of those oligosacchandes could be assigned easily.

• 出版日期2011-12
• 单位宁波大学